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Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants

Code: 9781786768636
William Gordon-Kamm, Pierluigi Barone, Sergei Svitashev, Jeffry D. Sander, Sandeep Kumar and Todd Jones, Corteva Agriscience, USA

Despite the conspicuous and rapid development of genome editing tools, implementing this technology in plants often remains constrained by our inability to regenerate fertile genome-modified plants. Finding a viable combination of delivery method, genome editing reagents, and plant regeneration system has often been both crop-specific and crop-limited. Recent progress using morphogenic genes such as Wuschel2 (Wus2) and Babyboom (Bbm) has alleviated some of these crop-specific challenges, and along with other improvements, continues to expand the range of plants that can be edited.

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DOI: 10.19103/AS.2020.0082.12
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Table of contents 1 Introduction 2 Delivery of genome editing components into plant cells 3 Delivery methods for genome editing reagents: delivery into single cells 4 Delivery methods for genome editing reagents: delivery into intact tissues 5 Alternatives to DNA delivery 6 Morphogenic genes increase transformation efficiency and extend genotype range 7 Morphogenic genes permit transformation in nontraditional explants 8 Future trends 9 Where to look for further information 10 References
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