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Advances in screening plants for edits and off-targets

Code: 9781801461405
Chun Wang and Kejian Wang, China National Rice Research Institute, China

Chapter synopsis: Genome editing technology greatly accelerates the development of plant molecular research and crop breeding. However, accurately detecting mutations induced by sequence-specific nucleases between large-scale plants produced from transformation become a challenge. This chapter introduces some frequently-used molecular methods of identify genome editing induced mutation or off-targets, which developed in recent years. It also discusses the advantages and disadvantages of these methods and the scope of application, providing a useful reference for researchers who will identify genome-edited plants.

DOI: 10.19103/AS.2020.0082.14
Table of contents 1 Introduction 2 PCR/RE assay 3 Mismatch cleavage assay 4 PCR/RNP 5 High-resolution melting (HRM) analysis 6 ACT-PCR 7 Analysis of Sanger sequencing chromatograms 8 Next generation sequencing (NGS) 9 Detection of off-target effects 10 Conclusion and future trends 11 Where to look for further information 12 References

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