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Advances in guide RNA design for editing plant genomes using CRISPR-Cas systems

Code: 9781801461382
Kaiyuan Chen, Hao Liu and Kabin Xie, Huazhong Agricultural University, China; Muhammad Tahir ul Qamar and Ling-Ling Chen, Huazhong Agricultural University, and Guangxi University, China

Chapter synopsis: This chapter discusses the general rules for selecting target sites for genome editing using the CRISPR-Cas technology and summarizes the bioinformatic tools that can be used to design sgRNA sequences.

DOI: 10.19103/AS.2020.0082.07
£25.00
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Table of contents 1 Introduction 2 Designing sgRNAs 3 Bioinformatics programs for designing sgRNAs 4 Predicting the outcome of CRISPR-mediated genome editing 5 Conclusion 6 Where to look for further information 7 References

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