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CRISPR/Cas9-mediated genome editing in Brassica

Code: 9781801461443
Cheng Dai, Xia Tian and Chaozhi Ma, Huazhong Agricultural University, China

Chapter synopsis: CRISPR/Cas9 is a valuable tool for both basic and applied research that has been widely applied to different plant species. In this chapter, we reviewed the application of CRISPR/Cas9 genome editing toolkit in Brassica crops. We also provided a case study in Brassica napus. Collectively, our results demonstrate that CRISPR/Cas9 is an efficient tool for creating targeted genome modifications at multiple loci in B. napus. These findings open many doors for biotechnological applications in oilseed crops.

DOI: 10.19103/AS.2020.0082.23
Table of contents 1 Introduction 2 Gene disruption in Brassica species 3 Homologous recombination-based editing in Brassica species 4 Case study: generating mutants by CRISPR/Cas9 toolbox 5 Conclusion and future trends 6 Where to look for further information 7 References

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