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Using phenotyping techniques to analyse crop functionality and photosynthesis

Code: 9781801465328
Eva Rosenqvist, University of Copenhagen, Denmark

Chapter synopsis: With climate change the occurrence of extreme weather events will increase, making stress resilience an essential trait for future crop breeding. Whilst we have a good understanding of the effect of single stress factors on photosynthesis, there is a knowledge gap on how combined stresses affect the photosynthesis. The combination of drought and heat is of special interest since they have opposing effects on stomatal regulation. Chlorophyll fluorescence can be applied in medium and high-throughput phenotyping and Fv/Fm can identify heat tolerant and sensitive genotypes, while low-throughput gas exchange can establish the mechanistic relationships that relate to harvest yield during stress. Most measurement of photosynthesis is done at steady state and saturating light after application of a single stress. Recent results indicate the importance of the way photosynthesis responds to combined stresses and fluctuating light. This chapter discusses how to perform analyses reflecting these more complex conditions.

DOI: 10.19103/AS.2022.0102.15
Table of contents 1 Introduction 2 Understanding photosynthesis and its relationship to crop growth and stress response 3 Phenotyping photosynthesis in varying environmental conditions 4 Using gas exchange to analyse photosynthesis 5 Using porometry and thermal imaging of gs and hyperspectral techniques 6 Using chlorophyll fluorescence 7 Photosynthesis and climate change: accounting for heat stress, drought stress and elevated CO2 8 Case studies 9 Conclusions 10 Where to look for further information 11 References 1 Introduction

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