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Site-directed genome modification

Code: 9781838798390
Jochen Kumlehn, Stefan Hiekel and Nagaveni Budhagatapalli, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany

Chapter synopsis: The principle of customized endonuclease-triggered genome modification offers unprecedented opportunities for the elucidation of gene functions and the improvement of crop plant performance. The advantage of this technology lies in the possibility of defining the genomic site where a modification is to be made. This chapter provides an overview of the principles of targeted mutagenesis, the state-of-the-art, current limitations, applications of the technology as well as future perspectives on the use of customizable endonucleases in research and breeding devoted to wheat and barley.

DOI: 10.19103/AS.2019.0051.30
Table of contents 1 Introduction 2 Major principles and formats of customizable endonucleases 3 Delivery of customized endonucleases to cells 4 Selection of target DNA motifs and validation of endonuclease components 5 Genetic modifications 6 Functional validation of gene functions and improvement of crop plant performance 7 Off-target mutations 8 Future trends and conclusion 9 Where to look for further information 10 References

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